4. A colleague wishes to separate a mixture of DNA fragments using gel electrophoresis and asks you if he can use alternating instead of direct current, because he has mislaid the DC equipment. How would you answer? Provide evidence.
I would advise against using alternating current for gel electrophoresis because it can cause the DNA fragments to move in the wrong direction and may result in inaccurate results. Gel electrophoresis requires a consistent flow of current in one direction in order to properly separate the DNA fragments based on size. Direct current is the standard and recommended method for gel electrophoresis to ensure accurate and reliable results. It is important to use the appropriate equipment and follow established protocols to ensure the success of the experiment.
