The three lines of defense, your body has against disease includes all except

the use of antibiotics.

The three lines of defense your body has against disease are physical barriers (such as skin and mucus membranes), the innate immune system (including white blood cells and inflammation), and the adaptive immune system (which produces antibodies and memory cells to fight off specific pathogens). Antibiotics are not a natural defense system of the body, but rather medications used to kill or inhibit the growth of bacteria.