Title: “Exploring the potential of Catharanthus Roseus for Phytoremediation of Heavy Metal Contamination: A Review”

The review of the scientific journal article titled "Uptake of Nickel and Lead in Catharanthus roseus Plants" provides valuable insights into the accumulation of heavy metals in plants and their potential for phytoremediation. Metals such as Zn, Cu, Mn, and Co are essential for plant growth, while heavy metals like Pb, Co, Cd, Ni, As, and Cr can have toxic effects on plants and animals. These heavy metals pose a threat to human and animal health due to their persistence in the environment (Tchounwou et al., 2012).
Phytoremediation involves using plants to remove, transfer, stabilize, or detoxify heavy metals in contaminated soil and water. Through processes like phytoextraction, phytostabilization, and phytovolatilization, plants can effectively reduce the levels of heavy metals in the environment. Certain plant species, known as hyperaccumulators, have the ability to accumulate high concentrations of heavy metals within their tissues. Phytoremediation is a cost-effective and sustainable approach to remediating contaminated sites compared to traditional methods. Research is ongoing to optimize phytoremediation techniques and identify the most suitable plant species for different types of heavy metal contamination. Overall, phytoremediation shows great potential for mitigating the impacts of heavy metal contamination on ecosystems and human health (Baker & Brooks, 1989) .
In the study on the uptake of Nickel and Lead in Catharanthus roseus plants, it was found that the plant species has a higher accumulation of metals in the roots compared to stems and leaves. Over a period of 60 days, Nickel accumulation increased in all parts of the plant, with high accumulation observed after 40 days. The Bioconcentration Factor (BCF) and Translocation Factor (TF) values indicated that Catharanthus roseus is a good accumulator of lead and nickel, showing potential for phytoremediation and phytostabilization of heavy metal-contaminated environments (Subhashini & Swamy, 2013).
The table and figure provided show the accumulation of lead in different parts of the Catharanthus roseus plant during the experimental period. The table presents the total accumulation of lead (in mg/kg) in the plant parts (leaf, stem, and root) at different time points (20th, 40th, and 60th day) of the experiment. It also includes the total accumulation of lead for each plant part and the overall total accumulation.
From the table, can observe the following: Leaf: The lead accumulation in the leaf remains relatively constant throughout the experimental period, with values ranging from 24.03 mg/kg to 24.95 mg/kg. The total accumulation in the leaf over the entire period is 0.92 mg/kg.
Stem: The lead accumulation in the stem increases over time, with values ranging from 60.69 mg/kg to 69.49 mg/kg. The total accumulation in the stem over the entire period is 8.8 mg/kg.
Root: The lead accumulation in the root shows a significant increase over time, with values ranging from 21.47 mg/kg to 88.81 mg/kg. The total accumulation in the root over the entire period is 67.34 mg/kg.
Total Accumulation: The total accumulation of lead in the plant (considering all plant parts) increases over time, with values ranging from 106.19 mg/kg to 183.33 mg/kg. The overall total accumulation over the entire period is 77.06 mg/kg.
Based on Table 2, the total accumulation of Nickel (mg/kg) in Catharanthus roseus during the experimental period is shown. The table provides data for different plant parts (leaf, stem, and root) at different time points (20th, 40th, and 60th day) of the experiment.
The "Total Accumulation" column represents the sum of Nickel accumulation in all plant parts for each time point. For example, on the 20th day, the total accumulation of Nickel in Catharanthus roseus is 10.49 mg/kg.
From the table, it can observe that the total accumulation of Nickel increases over time. On the 40th day, the total accumulation is 18.82 mg/kg, and on the 60th day, it further increases to 22.8 mg/kg.
Based on the information provided, about the accumulation of lead in Catharanthus roseus during the experimental period would be the option that mentions the specific plant part with the highest total accumulation of lead. Therefore, "Root" of the Catharanthus roseus plant, which accumulated a total of 67.34 mg/kg of lead over the entire experimental period.
"In the similar study, Qurban et al. (2021) found that metal accumulation profiles of Catharanthus roseus and Celosia argentea were provides valuable insights into the accumulation of heavy metals in plants, there are still unanswered questions concerning the long-term effects of metal accumulation in Catharanthus roseus plants and the transfer of metals to other organisms in the ecosystem. Future studies could focus on expanding the sample size, conducting long-term studies on the plant's efficiency in removing heavy metals, and exploring the ecological implications of using this plant for remediation.
In conclusion, the review of the scientific journal article sheds light on the potential of Catharanthus roseus plants for phytoremediation of heavy metal-contaminated environments. With further research and exploration of optimal conditions for using this plant species, it may offer a sustainable and environmentally friendly solution to mitigate the impact of heavy metal contamination on public health and ecosystems (Baby Tabassum, Hashim, & Bajaj, 2020).

Questions
Answer the Questions below using the information given above. Write at least three sentences for each questions and explain them.
In an attempt to review the article, answer the following questions below.
1. What is the purpose (aims/objectives) of the study?
2. What questions (research questions or hypotheses) were asked?
3. What was the sample size? Is this representative of the larger population?
4. Identify dependent variable and independent variable (if provided)? Was there a control?
5. Describe how the data was collected, what was the sample size? Is this a representative of the larger population?
6. What factors might have affected the outcome
7. What assumptions did the author make?
8. What were the major findings?
9. What surprised you or struck you as interesting?
10. What questions are still unanswered?
11. Do the data and conclusions contradict each other?
12. Is there sufficient data to support the author's generalizations?
13. What questions remain unanswered?
14. How could future studies be improved?

1. The purpose of the study was to investigate the potential of Catharanthus roseus plants for phytoremediation of heavy metal contamination in the environment. The study aimed to assess the accumulation of heavy metals, specifically lead and nickel, in different parts of the plant and determine its suitability for phytoremediation techniques.

2. The research questions asked in the study were focused on the uptake of lead and nickel in Catharanthus roseus plants, the distribution of these metals in different plant parts over time, and the potential for using this plant species in phytoremediation efforts.

3. The sample size and specific details regarding it were not provided in the information reviewed. However, it is essential to have a sufficiently large and diverse sample size to ensure the results are representative of the larger population.

4. The dependent variables in the study were the accumulation of lead and nickel in different plant parts, while the independent variables could include factors such as time, soil conditions, and metal concentrations. It is likely that there was a control group used in the experiment.

5. The data for the study was collected by analyzing the accumulation of lead and nickel in different parts of Catharanthus roseus plants at various time points (20th, 40th, and 60th day). The sample size and representativeness of the larger population were not specified in the information provided.

6. Factors that might have affected the outcome of the study could include the initial concentration of heavy metals in the soil, the growth conditions of the plants, the duration of the experiment, and the species-specific characteristics of Catharanthus roseus.

7. The authors may have made assumptions about the consistency of metal uptake and accumulation in the plant, the efficiency of Catharanthus roseus in phytoremediation, and the general applicability of the study findings to other heavy metal-contaminated environments.

8. The major findings of the study included the higher accumulation of lead and nickel in the roots of Catharanthus roseus plants compared to the stems and leaves, with a significant increase in metal accumulation over time. The plant showed a potential for phytoremediation and phytostabilization of heavy metal contaminants.

9. One interesting aspect of the study was the observation that the root of Catharanthus roseus accumulated the highest total amount of lead over the experimental period, highlighting the plant's ability to effectively uptake and retain heavy metals from the soil.

10. Some unanswered questions from the study include the long-term effects of metal accumulation in Catharanthus roseus plants, the transfer of metals to other organisms in the ecosystem, and the ecological implications of using this plant species for remediation.

11. The data and conclusions presented in the review do not seem to contradict each other, as the findings align with the potential of Catharanthus roseus plants for phytoremediation based on the accumulation of lead and nickel observed.

12. The information provided in the review does seem to support the author's generalizations about the potential of Catharanthus roseus for phytoremediation, but more detailed data and further research would be needed to make definitive conclusions.

13. Some questions that remain unanswered include the optimal conditions for using Catharanthus roseus in phytoremediation, the efficiency of the plant in removing heavy metals, and the broader ecological impacts of using this plant for remediation.

14. Future studies could be improved by expanding the sample size, conducting long-term research on the plant's phytoremediation efficiency, exploring the ecological implications in more detail, and investigating other potential plant species for remediation purposes.