A 4th year medical laboratory student wanted to study the prevalence of malaria among pregnant women for his graduation research. The student prepared 10percent Giemsa stain working solution to stain thin and thick blood films prepared from pregnant women via skin puncture. During examination, the student observed that few of the slides appeared to be too blue under light microscope. What is the most likely cause of the problem in this procedure? a. Inadequate washing of the stain b. Too high acidity of the stain c. Too thin films d. Insufficient staining time
The most likely cause of the problem in this procedure is d. Insufficient staining time. The slides appeared to be too blue because the staining process was not completed properly. This could be due to not letting the slides soak in the staining solution for the appropriate amount of time.
