You take 0.03 mo of the flowthrough fraction, measure the absorbable using the same conditions as in the standard curve above, and get a reading of 0.52. What is the protein concentration of the flowthrough fraction (mg/ml)? y=12.235x
To find the protein concentration of the flowthrough fraction, we can use the equation y = 12.235x, where y is the absorbance reading and x is the protein concentration in mg/ml.
Given that the absorbance reading is 0.52 and the protein concentration is 0.03 mo, we can substitute these values into the equation to solve for x.
0.52 = 12.235 * 0.03x
Dividing both sides of the equation by 12.235:
0.52 / 12.235 = 0.03x
0.0425 = 0.03x
Dividing both sides of the equation by 0.03:
0.0425 / 0.03 = x
x ≈ 1.4167
Therefore, the protein concentration of the flowthrough fraction is approximately 1.4167 mg/ml.
To find the protein concentration of the flowthrough fraction, we need to use the equation y = 12.235x, where y is the absorbance and x is the protein concentration.
Given that the absorbance of the flowthrough fraction is 0.52, we can plug this value into the equation and solve for x.
0.52 = 12.235x
To solve for x, divide both sides of the equation by 12.235:
x = 0.52 / 12.235
x ≈ 0.0425
So, the protein concentration of the flowthrough fraction is approximately 0.0425 mg/ml.
To calculate the protein concentration of the flowthrough fraction, we need to use the equation y = 12.235x, where y represents the absorbance value and x represents the protein concentration in mg/ml.
Given that the absorbance value is 0.52 and we need to solve for x, we can rearrange the equation as follows:
0.52 = 12.235x
Now we can solve for x by dividing both sides of the equation by 12.235:
x = 0.52 / 12.235
x ≈ 0.0425 mg/ml
Therefore, the protein concentration of the flowthrough fraction is approximately 0.0425 mg/ml.