how many templates does pcr require?

PCR (Polymerase Chain Reaction) typically requires two templates: one for the forward primer and another for the reverse primer. These templates are usually DNA molecules that flank the region of interest that will be amplified by PCR. In addition, PCR also requires other ingredients such as dNTPs (deoxyribonucleoside triphosphates), Taq polymerase, and buffer solutions to facilitate the amplification process.

An eyelash is stuck behind my upper eyelid. Is it going to stay there forever?

It is unlikely that the eyelash will stay there forever. Usually, the eyelash will naturally fall out or become dislodged and eventually work its way out of your eye. However, if the eyelash is causing discomfort, irritation, or a persistent feeling that something is in your eye, it is best to seek medical attention from an eye specialist. An ophthalmologist or optometrist can examine your eye to confirm the presence of the eyelash and remove it safely, minimizing the risk of injury or infection.

I cannot get it out, it is stuck between my upper eyelid and my eye!!

If the eyelash is stuck between your upper eyelid and your eye and you're unable to remove it, you should seek immediate medical attention from an eye specialist or an emergency room. It's important not to rub your eye or try to remove the eyelash yourself, as this can cause further irritation or damage to your eye. The specialist will perform a thorough examination and remove the eyelash safely using specialized tools. This will help to ensure that there is no further damage to your eye or infection.

will it go away naturally?

If an eyelash is stuck between your upper eyelid and eye, it is not likely to go away naturally. In most cases, the eyelash will have to be removed by a medical professional to prevent any damage or infection to the eye. It is important to seek medical attention promptly to avoid any complications. If left untreated, the eyelash can scratch the cornea or lead to an eye infection. These problems can be very harmful to the eye and may cause long-term damage to your vision. Therefore, it is always better to get medical help and have it removed by an eye specialist rather than waiting for it to go away on its own.

should i use eversion to get it out?

Eversion (turning of the eyelid) is a technique commonly used by eye specialists to remove foreign objects or debris from underneath the eyelid. However, it is not recommended to attempt eversion on your own to remove an eyelash. This can cause further irritation or damage to your eye and may even push the eyelash deeper into your eye.

Instead, it is recommended that you seek medical attention from an eye specialist or an emergency room to have the eyelash removed safely. They will have the expertise and knowledge to remove the eyelash without causing further damage or injury to your eye.

In the meantime, avoid rubbing your eye or trying to remove the eyelash yourself, as this can exacerbate the discomfort and cause further irritation.

should i get others to do it? i dont want to take a drive to the eye doctor just to get an eyelask out