Examine the plasmid below and the five gels and answer the questions that follow.
a.) Which of these gels correspond to an EcoR1 digest? why?
b) Which of these gels corresponds to a Sal1 disgust? Why?
i cant post urls but if you search this up youll find the diagram please help
To answer the questions, I need to consider the provided plasmid and the five accompanying gels. Unfortunately, you mentioned that you cannot post URLs or provide any specific diagrams or images. However, I can still explain the general process of identifying the corresponding gels based on EcoR1 and Sal1 digestion.
1. EcoR1 Digest:
An EcoR1 digest refers to the plasmid DNA being cut with the restriction enzyme EcoR1. EcoR1 recognizes a specific DNA sequence and cuts the DNA at that site. Typically, an EcoR1 digest results in linearized plasmid DNA.
To identify the gel corresponding to an EcoR1 digest:
- Look for a gel that shows linearized DNA fragments rather than circular forms.
- Linearized DNA would typically result in multiple bands of different sizes, representing the fragments resulting from EcoR1 cutting the DNA at multiple recognition sites.
2. Sal1 Digest:
A Sal1 digest refers to the plasmid DNA being cut with the restriction enzyme Sal1. Similar to EcoR1, Sal1 recognizes a specific DNA sequence and cuts the DNA at that site. Sal1 digestion may also result in linearized plasmid DNA or fragments of various sizes.
To identify the gel corresponding to a Sal1 digest:
- Look for a gel that shows linearized DNA fragments or fragments of various sizes, similar to the EcoR1 digest.
- The specific band patterns may differ from those in the EcoR1 digest, as Sal1 cuts at different recognition sites.
In order to provide a more accurate answer, it would be helpful to have access to the provided diagrams or additional information.