Write down the primer sequence, starting at position 5 of the following sequence, assuming it is 10bp in size. Also write down the 5’ end.
5' -ATGCCGATGTAGGGCGGGATGGAGAGATAGAGAGAGTCACAAT-3'
where should I start writing.
To find the primer sequence starting at position 5 in the given sequence, we need to count and locate the 5' end of the sequence. The 5' end is always written at the beginning of the sequence.
Let's count the nucleotides to find the 5' end:
5' -ATGCCGATGTAGGGCGGGATGGAGAGATAGAGAGAGTCACAAT-3'
Counting from left to right, we can see that the 5' end is at the very left, which corresponds to the start of the provided sequence. Therefore, you should start writing the primer sequence from the left, at the 5' end of the given sequence.
Now, to determine the primer sequence, we start at the 5' end and take the next 10 bases. Since the primer is defined as 10 base pairs (bp) in size, we can extract the sequence starting from position 5:
5' -ATGCCGATGTAGGGCGGGATGGAGAGATAGAGAGAGTCACAAT-3'
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The primer sequence starting from position 5 would be "CCGATGTAGG".
So, the primer sequence, starting at position 5, assuming it is 10bp in size, is "CCGATGTAGG".