This step gives an investigation a specific direction or focus.

A. Observation
B. Question
C. Hypothesis***
D. Analysis

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The correct answer is C. Hypothesis.

To determine the answer, we need to understand the steps in the scientific investigation process.

1. Observation: This is the initial step where you observe and gather information about a particular phenomenon or problem.

2. Question: Based on the observations, you develop a question or problem statement that you want to investigate further.

3. Hypothesis: Once you have formulated a question, you develop a hypothesis. A hypothesis is an educated guess or a possible explanation for the observed phenomenon. It gives the investigation a specific direction or focus. It usually takes the form of an "If...then..." statement, where you propose that if something specific occurs (the "If" part), then a particular outcome or effect will result (the "Then" part).

4. Analysis: After formulating a hypothesis, you proceed to collect data through experiments or observations. In the analysis phase, you examine and interpret the data to see if it supports or disproves your hypothesis.

So, the correct answer is C. Hypothesis, as it is the step that gives an investigation a specific direction or focus.