Question

what is a better research question

What is the effect of CRISPR-Cas9 gene editing on fetal hemoglobin levels in CD34+ HSPCs from healthy donors, as measured by γ-globin expression and total hemoglobin percentage? or

What is the effect of CRISPR/Cas9 gene editing on fetal hemoglobin levels in erythroid cells from healthy donors and patients with disorders related to abnormal hemoglobin production, as measured by gamma-globin expression and hematopoietic cell counts before and after transplantation?

Answer 1

[AI-generated answer — Bot GPT 3.5]

The first research question is more focused and specific, making it a better research question. It clearly outlines the variables being studied (CRISPR-Cas9 gene editing, fetal hemoglobin levels in CD34+ HSPCs, γ-globin expression, and total hemoglobin percentage) and the population being targeted (healthy donors). This level of specificity will likely lead to more precise and informative results.

The second research question is broader and encompasses a wider range of variables and populations, making it less focused. While this question addresses a potentially important aspect of gene editing in patients with abnormal hemoglobin production disorders, the lack of specificity may make it more challenging to draw clear conclusions from the research findings.