Write laboratory report in the following topic:

1. The Age estimation.

Title: Age Estimation of Sample Populations Using Chronological Markers

Abstract:
The aim of this study was to estimate the age of individuals in a sample population by analyzing chronological markers found within their biological samples. Special attention was given to dental records, bone density, and epiphyseal fusion-based techniques. By accurately estimating the age of individuals, this research aimed to improve the understanding of developmental processes, identify potential health concerns, and aid in the investigation of unidentified human remains. The results demonstrated significant correlations between the investigated markers and chronological age, supporting their efficacy in age estimation.

1. Introduction:
Age estimation plays a crucial role in various fields, such as forensic science, anthropology, and archeology. Identifying the age of individuals accurately is essential for determining personal identification, investigating crimes involving minors, assessing growth patterns, and understanding the demography of a population. This study aimed to investigate and validate various techniques commonly employed in age estimation based on dental records, bone density, and epiphyseal fusion.

2. Methods:
2.1 Sample Population:
A sample population consisting of 100 individuals, ranging in age from 10 to 80 years, was selected for this study. The selection criteria ensured individuals free from any known medical conditions that could affect the investigated markers.

2.2 Dental Records:
Dental records were collected for each individual, including tooth eruption patterns, tooth wear, and dental age indicators. Data collected were assessed by an experienced dental examiner to estimate the dental age of individuals using established standards and classification systems.

2.3 Bone Density:
Bone mineral density assessments were conducted using dual-energy x-ray absorptiometry (DXA) scans. Measurements were performed on the lumbar spine and femoral neck. The obtained values were compared to a reference database to obtain age estimates based on the categorization of bone mineral density percentiles.

2.4 Epiphyseal Fusion:
Radiographic images of the left hand and wrist were obtained for all individuals using standard techniques. The degree of epiphyseal fusion in the radius, ulna, and hand bones was analyzed using a scoring system based on established criteria. These scores were then correlated with age to estimate the chronological age of individuals.

3. Results:
The data obtained from the dental records, bone density measurements, and epiphyseal fusion scores were statistically analyzed to establish correlations with chronological age. The correlations were found to be significant (p < 0.05) for all investigated markers, indicating their effectiveness in age estimation.

4. Discussion:
The results of this study confirmed the reliability and validity of dental age estimation, bone density assessment, and epiphyseal fusion scoring as effective techniques for age estimation in populations. The findings align with previous studies and strengthen the existing body of knowledge in the field.

5. Conclusion:
Age estimation is an important aspect in various scientific disciplines, including forensic science, anthropology, and archeology. The present study successfully validated commonly employed techniques such as dental age estimation, bone density assessment, and epiphyseal fusion scoring in accurately estimating chronological age. These validated techniques can provide valuable information in personal identification, forensic investigations, and the study of human development. Further research utilizing larger sample sizes and exploring newer techniques should be conducted to enhance accuracy and precision in age estimation.