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1- the first step in manipulating and extracting DNA from cells is to _____ it into smaller pieces for analysis.

2- the second step in manipulating and extracting DNA from cells is to ______ it based on size using gel _______.

3- fragments of DNA separated by _______ and tagged with ______ can be used to determine the sequence of bases in a strand of DNA.

4- restriction _______ cut DNA at specific sites, which are like a key that fits only one ______.

5- a technique known as _____ sequencing searchers for matching sequences among the fragments and aligns them to reassemble the fragments and _______ the sequence.

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