in the preparation of the standard curve for protein analysis, 50mg BSA(bovine serum albumin) dissolved in water to a final volume of 5ml was used as stock solution.What is the weight of BSA in 0.1ml of stock solution? in 0.2ml?
.050g/5ml=w/.1ml
w=.001mg
Well, it seems like we've got ourselves a fun little math problem here, don't we? Let's give it a go!
First, let's find out the concentration of BSA in the stock solution. We started with 50 mg of BSA dissolved in 5 ml of water. So, to find the concentration, we divide the mass of BSA (50 mg) by the volume of the solution (5 ml):
Concentration = Mass/Volume
Concentration = 50 mg/5 ml = 10 mg/ml
Now, to figure out the weight of BSA in 0.1 ml of the stock solution, we multiply the concentration (10 mg/ml) by the volume:
Weight = Concentration x Volume
Weight = 10 mg/ml x 0.1 ml = 1 mg
Ta-da! So, in 0.1 ml of the stock solution, the weight of BSA is 1 mg.
Now, let's move on to 0.2 ml. Following the same steps:
Weight = Concentration x Volume
Weight = 10 mg/ml x 0.2 ml = 2 mg
Voilà! In 0.2 ml of the stock solution, the weight of BSA is 2 mg.
I hope that tickled your funny bone a little! If you have any more questions, just let me know!
To calculate the weight of BSA in a given volume of the stock solution, we can first determine the concentration of BSA in the stock solution. Then we can use the concentration to calculate the weight of BSA in the desired volume.
Given:
- Stock solution: 50 mg BSA dissolved in 5 ml of water
To determine the concentration of BSA in the stock solution, we need to divide the mass of BSA by the volume of the stock solution:
Concentration of BSA in the stock solution = (Mass of BSA) / (Volume of stock solution)
Concentration = 50 mg / 5 ml = 10 mg/ml
Now, we can calculate the weight of BSA in 0.1 ml and 0.2 ml of the stock solution.
For 0.1 ml of stock solution:
Weight of BSA = Concentration of BSA * Volume of stock solution
= 10 mg/ml * 0.1 ml
= 1 mg
For 0.2 ml of stock solution:
Weight of BSA = Concentration of BSA * Volume of stock solution
= 10 mg/ml * 0.2 ml
= 2 mg
Therefore, the weight of BSA in 0.1 ml of the stock solution is 1 mg, and the weight of BSA in 0.2 ml of the stock solution is 2 mg.
To calculate the weight of BSA in a given volume of the stock solution, we need to determine the concentration of BSA in the stock solution. The concentration of BSA can be calculated using the formula:
Concentration (C) = Mass (m) / Volume (V)
Given that the stock solution has a total volume of 5 mL and contains 50 mg of BSA, we can calculate the concentration as follows:
C = 50 mg / 5 mL
C = 10 mg/mL
Now, to find the weight of BSA in a specific volume of the stock solution:
For 0.1 mL:
Weight (m) = Concentration (C) x Volume (V)
Weight (m) = 10 mg/mL x 0.1 mL
Weight (m) = 1 mg
Therefore, the weight of BSA in 0.1 mL of the stock solution is 1 mg.
For 0.2 mL:
Weight (m) = Concentration (C) x Volume (V)
Weight (m) = 10 mg/mL x 0.2 mL
Weight (m) = 2 mg
Therefore, the weight of BSA in 0.2 mL of the stock solution is 2 mg.