How much solute is required to make 600mL of a 0.4 M solution of Tris buffer? FW of Tris Buffer= 121.1g
Work: Molarity equation= moles of solute/ liters of solution
Step 1: 121.1 g / 121.14 g/mol TB = 0.9996 mol TB
Step 2: Molarity Eq'n= moles of solute/ liters of sol'n ... so, 0.9996/0.6 L = 1.666 mole (M) of solute
OR isn't it just ... 0.4 M sol'n = moles of solute/0.6 L = 0.24 mol of solute ?
Wow, did you go a journey.
it is the "just...", .24mol of solute, and you can convert that to mass
.24mol*121g=29g
To calculate the amount of solute required to make 600 mL of a 0.4 M solution of Tris buffer, you can use the molarity equation: moles of solute/liters of solution.
Step 1: Convert the molecular weight of Tris buffer (FW = 121.1 g) to moles:
FW = 121.1 g/mol
Moles of Tris buffer = 121.1 g / 121.1 g/mol = 0.9996 mol
Step 2: Use the molarity equation to calculate the moles of solute required:
Molarity (M) = moles of solute / liters of solution
0.4 M = moles of solute / 0.6 L
So, rearranging the equation to solve for moles of solute, you get:
moles of solute = 0.4 M x 0.6 L = 0.24 mol
Therefore, you would need 0.24 moles of solute to make 600 mL of a 0.4 M solution of Tris buffer.
To calculate the amount of solute required to make a 0.4 M solution of Tris buffer in 600 mL of solution, you can use the molarity equation:
Molarity = moles of solute / liters of solution
Step 1: Calculate the moles of Tris buffer.
Given that the formula weight (FW) of Tris buffer is 121.1 g/mol, divide the given weight of Tris buffer (121.1 g) by its FW:
121.1 g / 121.1 g/mol = 0.9996 mol of Tris buffer
Step 2: Calculate the moles of solute required for the desired molarity.
Use the molarity equation:
0.4 M = moles of solute / 0.6 L
Rearrange the equation to solve for moles of solute:
moles of solute = 0.4 M x 0.6 L = 0.24 mol of solute
So, the amount of solute required to make a 0.4 M solution of Tris buffer in 600 mL is approximately 0.24 mol of solute.